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    可溶性高发酵日粮纤维和碳水化合物酶改善受F18 ETEC攻毒断奶仔猪的肠道屏障和生长性能

    发布单位:万博manbetx登录电脑版_万博manbetx官网客户端下载_manbetx万博官方下载

    查看次数:595

    时间:2019-05-27
    Qingyun Li,Eric R Burrough,Nicholas K Gabler,Crystal L Loving,Orhan Sahin,Stacie A Gould,John F Patience
    翻译:朱滔 

    本试验目的在于研究可溶性纤维和不可溶性纤维,以及碳水化合物酶(木聚糖,β葡聚糖和果胶酶)对被产肠毒素大肠杆菌(ETEC)攻毒仔猪腹泻率、免疫反应和生长性能的影响。

    试验选用60头断奶仔猪(6.9±0.1kg体重,23d),根据体重分为6组,单栏饲养,每组10个重复。试验处理包括:1个阴性对照组(无攻毒),1个攻毒对照组、甜菜渣组(攻毒+10%甜菜渣)、甜菜渣+酶组(攻毒+10%甜菜渣+碳水化合物酶) 、DDGS组(攻毒+15%DDGS)、DDGS+酶组(攻毒+15%DDGS+碳水化合物酶)。对照组日粮主要为玉米-豆粕型,添加13.5%小麦粉。这两种纤维以替代日粮中的淀粉添加。断奶后第7d口服6ml溶血性 F18 ETEC(约3.5×109 cfu/ml)或者6ml生理盐水。所有ETEC攻毒仔猪均为F18 ETEC易感基因型。14d试验期间所有仔猪自由采食和饮水。记录断奶后第0、7、14或15d的体重和采食量。攻毒第-7、0、1、2、3、5、7或8d收集仔猪粪便,评估溶血性大肠杆菌。攻毒后每天记录粪便评分,评估腹泻率。攻毒第-1、3、7或8d解剖时收集血液样品和肠道组织。

    试验数据显示,攻毒组猪攻毒后1-7d日增重和采食量显著低于对照组(P<0.05)。与攻毒组相比,甜菜渣+酶组攻毒前和攻毒后日增重显著升高(P<0.05)。DDGS组攻毒后腹泻率显著高于攻毒组(P<0.05)。甜菜渣组回肠大肠杆菌粘附程度显著低于攻毒组(P<0.05)。与攻毒组相比,甜菜渣+酶组攻毒后第三天触珠蛋白明显降低,DDGS+酶组C-反应蛋白显著降低(P<0.05)。与攻毒组相比,甜菜渣+酶组猪有较低的回肠肿瘤坏死因子α和较大的回肠阻塞素(ocln)mRNa((P<0.10),结肠ocln mRNa水平较高(P<0.05)。

    总之,DDGS提高了腹泻率和粪便中大肠杆菌脱落数。甜菜渣+酶改善仔猪攻毒后的生长性能,可能是由于降低了炎症因子。

    A soluble and highly fermentable dietary fiber with carbohydrases improved gut barrier integrity markers and growth performance in F18 ETEC challenged pigs

    This study aimed to evaluate the effects of a source of dietary soluble (SF) and insoluble fiber (IF) without or with exogenous carbohydrases (xylanase, β-glucanase, and pectinase) on diarrhea incidence, selected immune responses, and growth performance in enterotoxigenic Escherichia coli (ETEC)-challenged pigs. Sixty weaned pigs (6.9 ± 0.1 kg BW, ~23 d of age) were blocked by initial BW and placed in individual pens. Pens were randomly assigned to one of six treatments (n = 10 per treatment), including a nonchallenged control (NC), a positive challenge control (PC), the PC + a soluble fiber diet (10% sugar beet pulp) without (SF−) or with carbohydrases (SF+), and PC + an IF diet (15% corn distillers dried grains with solubles) without (IF−) or with carbohydrases (IF+). The control diet was primarily based on corn and soybean meal with 13.5% whey powder. The two sources of fiber were added at the expense of cornstarch in the control diet. Pigs were orally inoculated with 6 mL hemolytic F18 ETEC (~3.5 × 109 cfu/mL) or sham infected with 6 mL phosphate-buffered saline on day 7 (0 d postinoculation, dpi) postweaning. All ETEC challenged pigs were confirmed to be genetically susceptible to F18 ETEC. Pigs had free access to feed and water throughout the 14-d trial. Pig BW and feed intake were recorded on dpi −7, 0, and 7 or 8. Fecal swabs were collected on dpi −7, 0, 1, 2, 3, 5, and 7 or 8 to evaluate hemolytic E. coli shedding. Fecal score was visually ranked daily postchallenge to evaluate diarrhea incidence. Blood samples were collected on dpi −1, 3, and 7 or 8 at necropsy and intestinal tissues were collected at necropsy. Pigs on PC had lower dpi 1 to 7 ADG and ADFI than those on NC (P < 0.05). Compared with PC pigs, SF+ pigs had greater ADG during both pre- and postchallenge period (P < 0.05). The IF− increased postchallenge diarrhea incidence compared with PC (P < 0.05). Pigs on SF− had lower ileal E. coli attachment than PC (P < 0.05). The SF+ reduced haptoglobin and IF+ reduced C-reactive protein on dpi 3 compared with PC (P < 0.05). Compared with PC pigs, SF+ pigs tended to have lower ileal tumor necrosis factor alpha and greater ileal occludin (OCLN) mRNA (P < 0.10) and had greater (P < 0.05) colonic OCLN mRNA levels. Collectively, IF− increased incidence of diarrhea and fecal E. coli shedding compared with PC. The SF+ pigs had improved growth compared with PC pigs, likely due in part to a reduction in inflammatory intermediates.


    转自公众号:猪营养国际论坛

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